Confocal microscopy and immunofluorescence protocols, optimization methods and techniques - is there an immunofluorescence or confocal microscopy protocol you want to share? Do you have some comments, protocols or suggestions regarding deconvolution or scientific imaging tips? We want your feedback.
Protocols: immunofluorescence, cryostat sections, sample preparation ...
Troubleshooting:weak signal, high background noise? Try some of these suggestions.
Confocal microscopy (Laser Scanning Microscopy) differs from traditional widefield optical microscopy. For the research scientist, there are several advantages to using a confocal microscope to obtain data compared with conventional optical microscopy. One of the hallmark features of this process is the ability to obtain serial optical sections with a confocal microscope. Using digital image processing techniques, these serial images can be re-assembled to form 3D representations of the structures being studied.
By applying various antibodies conjugated with fluorescent markers, the relative distributions of epitopes of interest can be studied. With the advancement of the methods and devices available, double and triple labeling techniques are now commonplace.
Confocal microscopy.org offers protocols, buffers, troubleshooting and other confocal microscopy resources.
______
Free full text research papers that use confocal-microscopy will be available soon.