confocal microscopy.org - confocal microscopy & related protocols:

Confocal microscopy and immunofluorescence protocols, optimization methods and techniques - is there an immunofluorescence or confocal microscopy protocol you want to share? Do you have some comments, protocols or suggestions regarding deconvolution or scientific imaging tips? We want your feedback.

Protocols: immunofluorescence, cryostat sections, sample preparation ...

Troubleshooting:weak signal, high background noise? Try some of these suggestions.

Buffers: permeabilization solution, PBS, tissue fixation ...

Concepts:definitions, explanations..

Links: a collection of useful microscopy and science resources

Confocal Microscopy & Related Protocols:

1. Immunofluorescence

2. Poly L-lysine coating coverslips and slides

3. Acid Washing coverslips and slides

4. Sample Preparation and Cryosectioning

Confocal microscopy safety: Please follow the safety recommendations supplied with the products or equipment you use, as well as state and institutional guidelines. The protocols and suggestions on this website are not a substitute for formal training. Confocal microscopy employs lasers - please follow proper protocol.