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| Green
Fluorescent Protein |
The green fluorescent protein (GFP) is a protein
from the jellyfish Aequorea victoria and the sea
pansy that fluoresces green when exposed to blue
light. In cell and molecular biology, its gene is
frequently used as a reporter of expression. In
modified forms it has been used to make biosensors.
The wild type GFP from Aequorea victoria has a major
excitation peak at a wavelength of 395 nm and a
minor one at 475 nm. Its emission peak is at 509
nm which is in the lower green portion of the visible
spectrum. The GFP from the sea pansy has a single
major excitation peak at 498 nm.
Some mutants of the GFP gene have been produced
with increased fluorescence. The major excitation
peak has been shifted to 490 nm with the peak emission
kept at 509 nm.
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