Confocal microscopy and immunofluorescence protocols, optimization methods and techniques - is there an immunofluorescence or confocal microscopy protocol you want to share? Do you have some comments, protocols or suggestions regarding deconvolution or scientific imaging tips? We want your feedback.
Protocols: immunofluorescence, cryostat sections, sample preparation ...
Troubleshooting:weak signal, high background noise? Try some of these suggestions.
Acid washing coverslips and slides improves adhesion of cells or polyamines/laminin
1. Heat coverslips/slides in a loosely covered beaker containing 1M HCl at 50-60oC for 8 -16 hours.
2. Allow to cool
3. Wash coverslips vigorously in ddH20
4. Rinse coverslips in 95% ethanol and let dry
note: Coverslips can be sterilised by UV irradiation before plating cells
Coverslips and slides can now be coated with Poly-L-lysine