Confocal microscopy and immunofluorescence protocols, optimization methods and techniques - is there an immunofluorescence or confocal microscopy protocol you want to share? Do you have some comments, protocols or suggestions regarding deconvolution or scientific imaging tips? We want your feedback.
Protocols: immunofluorescence, cryostat sections, sample preparation ...
Troubleshooting:weak signal, high background noise? Try some of these suggestions.
Poly L-lysine coated coverslips and slides provide higher adhesion,
reducing the chances of tissue or cell loss during processing.
1.
Use acid washed coverslips or slides (acid
washing protocol)
2. Poly L-lysine Stock solution: 0.1% (w/v in water). Store at room
temperature
3. Working poly L-lysine solution: dilute stock 1:10 in ddH2O. Store
for up to 2 months at 4 deg C.
4.
Incubate acid washed coverslips in the poly L-ysine for 5 minutes at
room temperature.
5. Remove the poly L-lysine with suction and leave to dry