Confocal microscopy and immunofluorescence protocols, optimization methods and techniques - is there an immunofluorescence or confocal microscopy protocol you want to share? Do you have some comments, protocols or suggestions regarding deconvolution or scientific imaging tips? We want your feedback.
Protocols: immunofluorescence, cryostat sections, sample preparation ...
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1. PubMed: PubMed, a service of the National Library of Medicine, includes over 15 million citations for biomedical articles back to the 1950's.
2. Immunofluorescence Method: This page provides a good introduction to some of the principles at work in immunofluorescent labelling.
3. Table of Fluorochromes: This is a table of some characteristics of fluorochromes useful for flow cytometry or fluorescence microscopy.
4. Double immunofluorescence staining for BCL-6: Any type of tissue is suitable for this technique, as long as the antigenicity for your antigen(s) is preserved. Use cytocentrifuged cells or frozen or paraffin dewaxed sections. Presence of endogenous fluorescent substances may affect your staining.
5. How does a confocal microscope work?: An explanation by Dr. E. Weeks. Includes sketches and basic principles.
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